W01 - Voltage imaging and beyond, next-generation molecular and optical tools for high-speed brain imaging (10:00-13:00)

Voltage imaging is starting to enable optical recording of subthreshold and spiking activity from ensembles of cells in behaving animals, a technology that holds promise to transform research in cellular and systems neuroscience. This progress is a result of technological leaps in the development of Genetically Encoded Voltage Indicators (GEVIs), and advancements in high-speed microscopy. The goal of this workshop is to bring together world leaders in protein engineering, microscope development and early adopters of the technology. These experts will present the latest developments in both GEVIs and high-speed microscopy, their integration to answer important questions in systems neuroscience, and discuss outstanding challenges. Labs of Eric Schreiter, Robert Campbell, and Adam Cohen stand behind the development of revolutionary GEVIs with enhanced brightness and sensitivity. Stephane Dieudonne and Adam Cohen develop unique solutions for one-photon and two-photon high-speed microscopy. Adam Cohen and Xue Han routinely use GEVIs to study neuronal activity in multiple brain regions in behaving mice. Yoav Adam and Ahmed Abdelfattah have led projects spanning the spectrum of this workshop, and after the talks they will lead a panel discussion on outstanding challenges. Together, this workshop will introduce the current state-of-the-art in the field. Voltage imaging is a “hot topic” and we are confident that this workshop will attract a lot of interest from FENS attendees.

Moderators:
-Yoav Adam, The Hebrew University of Jerusalem, Israel
-Ahmed Abdelfattah, Brown University, USA

Speakers: 
-Adam Cohen, Harvard University, USA – Voltage imaging in live mice
-Eric Schreiter, Howard Hughes Medical Institute, USA – Chemigenetic indicators of neuronal activity
-Robert E. Campbell, The University of Tokyo, Japan – Pushing the wavelength frontier for genetically encoded biosensors of neural activity
-Xue Han, Boston University, USA – Voltage imaging analysis of single neuron membrane dynamics during behavior
-Stéphane Dieudonne, PSL University, France – A light steering strategy for two-photon voltage imaging of neuronal activity in depth